David Wong Lab
Imagine a world where a routine visit to the dentist saves your life. Imagine a world where doctors do not have to draw your blood to test if you are sick. Imagine a world where procedures save countless lives and save billions of dollars. The goal of our research is to make this dream a reality. Here at the Wong Lab, we are pioneering research in the usage of saliva as a diagnostic medium for early detection and monitoring of systemic diseases. Additionally, we are focused on the fundamental biology of molecular markers in biofluids in particular their biogenesis, transport, metabolism, and their downstream effect on the pathophysiology of the human body, in health and disease.
W Lab Weblink: https://wlabucla.squarespace.com/welcome
Current Projects and Grants
Liquid Biopsy Detection of Non-Small-Cell Lung Carcinoma: Liquid biopsy has found its way into the cancer lexicon as a reference to tumor biomarkers within blood or other readily accessible biospecimens such as saliva that reflect the presence and biology of cancer. This project interleaves the expertise of lung cancer biologists, clinicians, and biostatisticians with industry engineers, converging on a novel liquid biopsy technology "EFIRM-Liquid biopsy (eLB)" that has already shown high sensitivity detecting circulating DNA mutations in patients with EGFR-mutant lung cancers. To address early lung cancer detection we are developing and validating independent assay for circulating tumor DNA (ctDNA) and microRNA in mutations commonly observed in lung cancer. This integrated platform will be validated in patients with screen- or incidentally- detected lung nodules in the size ranges that are most diagnostically challenging. Our overall research proposition is that blood-based biomarkers using the eLB-Lung Cancer Detection Panel (eLB-LCDP) will inform the accurate and robust classification of nodules as benign or malignant. Additionally, we are examining the unique physical characteristics of ctDNA in plasma and saliva and using state-of-the-art techqniues and methods.
Supporting Grants: U01 CA233370 (EFIRM-Liquid Biopsy (eLB): Ultrasensitive ctDNA and miRNA Detection for Early Assessment of Lung Cancer), UH2/UH3 CA206126 (Advancing EFIRM-Liquid Biopsy (eLB) to a CLIA-Certified Laboratory Developed Test (eLB-LDT) for Detection of Actionable EGFR Mutations in NSCLC Patients, and 1R21CA239052 (Ultra-short circulating tumor DNA (uctDNA) for liquid biopsy of non-small cell lung cancer).
Salivary Gastric Extracellular RNA Biomarkers for Gastric Cancer Detection: Extracellular RNA (exRNA) is an emerging paradigm as endocrine signals in cellular communication, biomarker development, therapeutic applications and systemic physiology. Our laboratory first reported the existence of a transcriptome and microRNA profile in cell free saliva followed by its scientific characterizations and clinical utilities including biomarker development for molecular oncology applications. Most recently we have performed RNA-sequencing in cell free saliva and reported three major types of RNA in saliva (mRNA, miRNA and snoRNA). We are interesting in testing the hypothesis that salivary extracellular RNA (exRNA) can be developed for the clinical detection of human diseases using gastric cancer as a model disease. Additionally we seek to improve and optimize current methods for salivary-exosome isolation which have been shown to be the vesicular transporter that houses exRNA cargo.
Supporting Grants: 1UG3/UH3 TR002978 (Acoustofluidic Separation (AFS), Purification and Raman Spectral Fingerprinting of Single EVs: From Cell of Origin to Target Cell and Biofluids), UH2/UH3 TR000923 (Clinical Utility of Salivary Extracellular RNA Biomarkers for Gastric Cancer Detection), and Ronnie James Dio “Stand UP and Shout” Cancer fund.
Assessing piRNAs in Oral Wound Healing: The objective of this project is to identify epigenetic alterations associated with accelerated oral wound healing phenotype in human oral keratinocytes. Using next-generation bisulfite sequencing, differential transcriptomic analysis, and bioinformatics analysis, we aim to identify the mechanism of oral specific piwi-interacting RNA (oral-piRNAs) in oral keratinocytes. BLAST results demonstrated oral-piRNAs mapping to transposable elements, specifically Alu. Knockdown of oral-piRNAs resulted in altered Alu methylation. Current findings suggest oral-piRNAs as intrinsic factors that modulate molecular pathways in human oral keratinocytes through de novo methylation of Alu elements.
Supporting Grants: F30 DE027615 (Oral-piRNAs Enhance Re-epithelialization in Oral Wound Healing).
- 1. Wang Z, Li F, Rufo J, Chen C, Yang S, Li L, Zhang J, Cheng J, Kim Y, Wu M, Abemayor E, Tu M, Chia D, Spruce R, Batis N, Mehanna H, Wong DTW, Huang TJ. Acoustofluidic Salivary Exosome Isolation: A Liquid Biopsy Compatible Approach for Human Papillomavir
- Murillo OD, Thistlethwaite W, Rozowsky J, …Wu C, Wong DTW, Galas DJ, Van Keuren-Jensen K, Patel T, Jones JC, Das S, Cheung KH, Pico AR, Su AI, Raffai RL, Laurent LC, Roth ME, Gerstein MB, Milosavljevic A. exRNA Atlas Analysis Reveals Distinct Extracell
- Wei F, Strom C, Cheng J, Lin C, Hsu C, Guy S, Chia D, Kim Y, Li F, Elashoff D, Gorgan T, Tu M, Wei L, Xian R, Grody W, Su W, Wong D. EFIRM Liquid Biopsy (eLB) for Non-Invasive Early Lung Cancer Assessment. J Mol Diagn. 2018;20(6):738-742. PubMed PMID:
- Li F, Kaczor-Urbanowicz KE, Sun J, Majem B, Lo HC, Kim Y, Koyano K, Rao SL, Kang SY, Kim SM, Kim KM, Kim S, Chia D, Elashoff D, Grogan TR, Xiao X, Wong DTW. Characterization of Human Salivary Extracellular RNA by Next-generation Sequencing. Clin Chem.
- Wei F, Lin CC, Joon A, Feng Z, Troche G, Lira ME, Chia D, Mao M, Ho CL, Su WC, Wong DT. Noninvasive Saliva-based EGFR Gene Mutation Detection in Patients with Lung Cancer. Am J Respir Crit Care Med. 2014;190(10):1117-26. PubMed PMID: 25317990.
List of collaborators within and outside of UCLA:
- Denise Aberle, MD (UCLA Radiology)
- Eliott Abemayor, MD, PhD (UCLA Otolaryngology / Head and Neck Surgery)
- David Chia, PhD (UCLA Pathology)
- David Elashoff PhD (UCLA Biostatistics)
- Udayan Guha MD, PhD (NCI Clinical Research Center)
- Alexander Hoffmann PhD (UCLA)
- Chung-Liang Ho MD, PhD (National Cheng Kung University, Pathology)
- Tony Huang, PhD (Duke University Biomedical Engineering)
- Yong Kim, PhD (UCLA Dentistry)
- Kostyantyn Krysan PhD (UCLA Pulmonary Medicine)
- Matteo Pellegrini PhD (UCLA)
- Marilene Wang, MD (UCLA Otolaryngology / Head and Neck Surgery)
- Charles Strom, MD, PhD (Liquid Dx)
- Ya-Hong Xie PhD (UCLA Engineering)
- Guy Soo Hoo MD (GLA)
- Yeong-Wook Song MD, PhD (Seoul National University, Rheumatology)
- Wu-Chou Su MD, PhD (National Cheng Kung University, Thoracic Oncology)
- Arjan Vissink DMD, MD, PhD (Univeristy Medical Center Groningen)
List of Lab Members:
- David Wong, DMD, DMSc, Principal Investigator
- Feng Li, PhD, Project Scientist
- Taichiro Nonaka, DDS, PhD, Adjunct Assistant Professor
- Fang Wei, PhD, Adjunct Associate Professor
- Karolina Kaczor-Urbanowics, DDS, PhD, Project Scientist
- Samantha Chiang, DDS, DDS/PhD Student
- Jordan Cheng, DMD, PhD Student
Come join us for our weekly lab meetings and get to know more about what we do!